Identification of enteropathogenic and enterohaemorrhagic Escherichia coli strains by immunoserological detection of intimin.
J Appl Microbiol
; 108(3): 878-887, 2010 Mar.
Article
in En
| MEDLINE
| ID: mdl-19709337
ABSTRACT
AIMS:
To evaluate the sensitivity and specificity of polyclonal and monoclonal antibodies (Mabs) against intimin in the detection of enteropathogenic and enterohaemorrhagic Escherichia coli isolates using immunoblotting. METHODS ANDRESULTS:
Polyclonal and Mabs against the intimin-conserved region were raised, and their reactivities were compared in enteropathogenic E. coli (EPEC) and enterohaemorrhagic E. coli (EHEC) isolates using immunoblotting analysis. In comparison with rat antiserum, rabbit anti-intimin IgG-enriched fraction had a stronger recognition pattern to a wide spectrum of intimin types in different EPEC and EHEC serotypes. On the other hand, murine monoclonal IgG2b specific to intimin, with dissociation constant of 1.3x10(-8) mol l(-1), failed in the detection of some of these isolates.CONCLUSION:
All employed antibodies showed 100% specificity, not reacting with any of the eae-negative isolates. The sensitivity range was according to the employed antisera, and 97% for rabbit anti-intimin IgG-enriched fraction, followed by 92% and 78% sensitivity with rat antisera and Mab. SIGNIFICANCE AND IMPACT OF THE STUDY The rabbit anti-intimin IgG-enriched fraction in immunoblotting analysis is a useful tool for EPEC and EHEC diagnoses.
Full text:
1
Collection:
01-internacional
Health context:
3_ND
Database:
MEDLINE
Main subject:
Adhesins, Bacterial
/
Escherichia coli Proteins
/
Enteropathogenic Escherichia coli
/
Enterohemorrhagic Escherichia coli
/
Antibodies, Monoclonal
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Animals
Language:
En
Journal:
J Appl Microbiol
Year:
2010
Document type:
Article