Cellular uptake and toxicity of microparticles in a perspective of polymyxin B oral administration.
Int J Pharm
; 385(1-2): 42-6, 2010 Jan 29.
Article
in En
| MEDLINE
| ID: mdl-19837148
ABSTRACT
Alginate/chitosan microparticles with a mean size less than 1 microm, designed in a previous work for the targeting of polymyxin B to M-cells and, then, to the lymphatic system, were assayed for transport ability by enterocytes. Caco-2 cell monolayer model, combined with confocal microscopy, showed that microparticles were endocytosed by the cells through an energy-dependent process, being the process saturable at 6 h incubation. Furthermore, microparticles maintained the biological activity of the antibiotic and decreased the antibiotic cytotoxicity against Vero cell cultures. Therefore, simultaneous pathways via both M-cells and enterocytes could be proposed for such a microparticulate carrier.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polymyxin B
/
Drug Carriers
/
Enterocytes
/
Chitosan
/
Alginates
/
Endocytosis
/
Anti-Bacterial Agents
Limits:
Animals
/
Humans
Language:
En
Journal:
Int J Pharm
Year:
2010
Document type:
Article