Assessment of a robust model protocol with accelerated throughput for a human recombinant full length estrogen receptor-alpha binding assay: protocol optimization and intralaboratory assay performance as initial steps towards validation.

Freyberger, Alexius; Wilson, Vickie; Weimer, Marc; Tan, Shirlee; Tran, Hoai-Son; Ahr, Hans-Jürgen

Freyberger, Alexius; Wilson, Vickie; Weimer, Marc; Tan, Shirlee; Tran, Hoai-Son; Ahr, Hans-Jürgen.

Affiliation

Freyberger A; Bayer Schering Pharma AG, BSP-GDD-GED-GTOX Special Toxicology, Wuppertal, Germany. alexius.freyberger@bayerhealthcare.com

Reprod Toxicol ; 30(1): 50-9, 2010 Aug.

Article in En | MEDLINE | ID: mdl-20074635

Subject(s)

Animal Testing Alternatives; Biological Assay/methods; Endocrine Disruptors/pharmacology; Estrogen Receptor alpha/metabolism; Models, Biological; Recombinant Proteins/metabolism; Binding, Competitive; Biological Assay/standards; Dose-Response Relationship, Drug; Estrogen Receptor alpha/chemistry; Humans; Ligands; Protein Binding; Radioligand Assay; Recombinant Proteins/chemistry; Reproducibility of Results

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biological Assay / Recombinant Proteins / Estrogen Receptor alpha / Endocrine Disruptors / Animal Testing Alternatives / Models, Biological Type of study: Prognostic_studies Limits: Humans Language: En Journal: Reprod Toxicol Year: 2010 Document type: Article

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