Gene expression and distribution of key bone turnover markers in the callus of estrogen-deficient, vitamin D-depleted rats.
Calcif Tissue Int
; 87(1): 77-89, 2010 Jul.
Article
in En
| MEDLINE
| ID: mdl-20495792
ABSTRACT
An experimental rat model was used to test the hypothesis that in osteoporosis (OP) the molecular composition of the extracellular matrix in the fracture callus is disturbed. OP was induced at 10 weeks of age by ovariectomy and a vitamin D(3)-deficient diet, and sham-operated animals fed normal diet served as controls. Three months later a closed tibial fracture was made and stabilized with an intramedullary nail. After 3 and 6 weeks of healing, the animals were killed and the fracture calluses examined with global gene expression, in situ mRNA expression, and ultrastructural protein distribution of four bone turnover markers osteopontin, bone sialoprotein, tartrate-resistant acid phosphatase, and cathepsin K. Global gene expression showed a relatively small number of differently regulated genes, mostly upregulated and at 3 weeks. The four chosen markers were not differently regulated, and only minor differences in the in situ mRNA expression and ultrastructural protein distribution were detected. Gene expression and composition of fracture calluses are not generally disturbed in experimental OP.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Osteoporosis
/
Bony Callus
/
Biomarkers
/
Gene Expression
/
Fractures, Bone
Limits:
Animals
Language:
En
Journal:
Calcif Tissue Int
Year:
2010
Document type:
Article