Modeling nanopores for sequencing DNA.
Methods Mol Biol
; 749: 317-58, 2011.
Article
in En
| MEDLINE
| ID: mdl-21674382
Using nanopores to sequence DNA rapidly and at a low cost has the potential to radically transform the field of genomic research. However, despite all the exciting developments in the field, sequencing DNA using a nanopore has yet to be demonstrated. Among the many problems that hinder development of the nanopore sequencing methods is the inability of current experimental techniques to visualize DNA conformations in a nanopore and directly relate the microscopic state of the system to the measured signal. We have recently shown that such tasks could be accomplished through computation. This chapter provides step-by-step instructions of how to build atomic scale models of biological and solid-state nanopore systems, use the molecular dynamics method to simulate the electric field-driven transport of ions and DNA through the nanopores, and analyze the results of such computational experiments.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA
/
Sequence Analysis, DNA
/
Nanopores
Language:
En
Journal:
Methods Mol Biol
Year:
2011
Document type:
Article