Immunohistochemical characterization of rat thymic non-lymphoid cells. II. Macrophages and granulocytes defined by monoclonal antibodies.
Immunology
; 69(3): 416-22, 1990 Mar.
Article
in En
| MEDLINE
| ID: mdl-2179133
ABSTRACT
A panel of monoclonal antibodies (mAb) raised to antigens of rat thymic non-lymphoid cells (predominantly macrophages and granulocytes) was immunohistochemically characterized. Based on their staining patterns on cryostat thymic sections and double labellings using acid phosphatase activity, anti-cytokeratin mAb to exclude binding to epithelium or ED1 and ED2 mAb, specific for rat macrophages, antibodies were subdivided into four groups (i) R-MC 39 mAb strongly reactive with macrophages in the cortex and cortico-medullary zone (CMZ) and weakly with some scattered macrophages in the medulla, blood vessels and thymocytes; (ii) R-MC 40, 41 and 42 mAb specific for cortical macrophages and most CMZ macrophages; (iii) R-MC 43 and 44 mAb predominantly recognizing CMZ and medullary macrophages; (iv) R-MC 45 mAb strongly labelling granulocytes and weakly a subset of macrophages throughout the thymus and isolated cells in the medulla. The obtained results show considerable heterogeneity within mobile thymic non-lymphoid cells and the presence of specific or common antigens in macrophages of particular topographic localization in the rat thymus.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Thymus Gland
/
Granulocytes
/
Macrophages
/
Antibodies, Monoclonal
Limits:
Animals
Language:
En
Journal:
Immunology
Year:
1990
Document type:
Article