[Study on methods of deproteinization from Phellinus baumii polysaccharide].
Zhong Yao Cai
; 34(2): 293-5, 2011 Feb.
Article
in Zh
| MEDLINE
| ID: mdl-21823491
ABSTRACT
OBJECTIVE:
To study and compare different methods and influencing factors of deproteinization from Phellinus baumii polysaccharide.METHODS:
Sevag method and TCA method were used to remove protein from crude polysaccharide of Phellinus baumii, respectively.RESULTS:
The effect of TCA method on removing protein was superior to that of Sevag method,and the optimum conditions of removing protein with TCA were as follows 30 min treating time, 5% trichloroacetic acid,3 times treatemt. Under these conditions the rate of protein-removed was 82% and the rate of polysaccharide-lost was 10.8%.CONCLUSION:
TCA method is the optimal mean for deproteinization from Phellinus baumii polysaccharide.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polysaccharides
/
Basidiomycota
/
Proteins
Language:
Zh
Journal:
Zhong Yao Cai
Year:
2011
Document type:
Article