In vivo imaging of bile accumulation and biliary infarction after common bile duct ligation in rats.
Eur Surg Res
; 47(4): 240-7, 2011.
Article
in En
| MEDLINE
| ID: mdl-22056525
ABSTRACT
Obstructive cholestasis is caused by mechanical constriction or occlusion leading to reduced bile flow. Serious complications such as jaundice and even death may follow. Little is known about the initial phase of cholestasis and its consequences for the hepatic microarchitecture. This in vivo study aimed to characterize the nature and kinetics of developing obstructive cholestasis and focused on areas with biliary stasis and infarction by visualizing the autofluorescence of bile acids using intravital microscopy of the liver over a period of 30 h after bile duct ligation in rats. The innovation resided in performing fluorescence microscopy without applying fluorescent dyes. In animals subjected to obstructive cholestasis, the most significant changes observed in vivo were the concomitant appearance of (1) areas with bile accumulation increasing in size (6 h 0.163 ± 0.043, 18 h 0.180 ± 0.086, 30 h 0.483 ± 0.176 mm(2)/field) and (2) areas with biliary infarction (6 h 0.011 ± 0.006, 18 h 0.010 ± 0.004, 30 h 0.010 ± 0.050 mm(2)/field) as well as (3) a relation between the formation of hepatic lesions and enzyme activity in serum. The sequential in vivo analysis presented herein is a new method for the in vivo visualization of the very early changes in the hepatic parenchyma caused by obstructive cholestasis.
Full text:
1
Collection:
01-internacional
Health context:
6_ODS3_enfermedades_notrasmisibles
Database:
MEDLINE
Main subject:
Cholestasis
/
Liver
/
Microscopy, Fluorescence
Limits:
Animals
Language:
En
Journal:
Eur Surg Res
Year:
2011
Document type:
Article