Structure and mechanism of a cysteine sulfinate desulfinase engineered on the aspartate aminotransferase scaffold.

Fernandez, Francisco J; de Vries, Dominique; Peña-Soler, Esther; Coll, Miquel; Christen, Philipp; Gehring, Heinz; Vega, M Cristina

Fernandez, Francisco J; de Vries, Dominique; Peña-Soler, Esther; Coll, Miquel; Christen, Philipp; Gehring, Heinz; Vega, M Cristina.

Affiliation

Fernandez FJ; Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (Spanish National Research Council, CSIC), Madrid, Spain.

Biochim Biophys Acta ; 1824(2): 339-49, 2012 Feb.

Article in En | MEDLINE | ID: mdl-22138634

Subject(s)

Aspartate Aminotransferases/chemistry; Carbon-Sulfur Lyases/chemistry; Carbon-Sulfur Lyases/genetics; Catalytic Domain/genetics; Amino Acid Sequence; Amino Acid Substitution; Amino Acids, Dicarboxylic/metabolism; Aspartate Aminotransferases/genetics; Aspartate Aminotransferases/metabolism; Biocatalysis; Carbon-Sulfur Lyases/metabolism; Crystallography, X-Ray; Escherichia coli; Hydrogen Bonding; Ligands; Models, Molecular; Molecular Sequence Data; Protein Conformation; Protein Engineering; Pyridoxal Phosphate/chemistry; Pyridoxal Phosphate/metabolism; Pyridoxamine/analogs & derivatives; Pyridoxamine/chemistry; Pyridoxamine/metabolism; Substrate Specificity

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Full text: 1 Collection: 01-internacional Health context: 3_ND Database: MEDLINE Main subject: Aspartate Aminotransferases / Carbon-Sulfur Lyases / Catalytic Domain Language: En Journal: Biochim Biophys Acta Year: 2012 Document type: Article

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