Recombinant Fc-IL-18BPc isoform inhibits IL-18-induced cytokine production.
Hybridoma (Larchmt)
; 31(2): 99-104, 2012 Apr.
Article
in En
| MEDLINE
| ID: mdl-22509913
ABSTRACT
IL-18 is a pro-inflammatory cytokine that is produced from T cells and NK cells. IL-18 has been implicated in the pathogenesis of various inflammatory and cardiovascular diseases. IL-18 binding protein (IL-18BP) is a natural inhibitor of IL-18 that possesses higher affinity to IL-18 than that of the IL-18 receptor alpha chain on the cell surface. Human isoform a and c among four isoforms of IL-18BPs have an inhibitory effect on IL-18-induced cytokines whereas mouse IL-18BP isoforms exist only in two isoforms c and d. Fc-fusion protein is a molecule in which the immunoglobulin Fc is fused genetically to a protein of interest, such as an extracellular domain of a receptor, ligand, or enzyme. In this study, we expressed and purified human Fc-IL-18BPa and c isoforms from CHO-DG44 cells and their biological activities were compared to each other. This is the first time that expressed recombinant human Fc-IL-18BPc has been examined for its biological activity on IL-18-induced IFNγ in human PBMC and IL-6 in A549/IL-18Rß.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Recombinant Fusion Proteins
/
Immunoglobulin Fc Fragments
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Interleukin-6
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Interferon-gamma
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Interleukin-18
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Intercellular Signaling Peptides and Proteins
Limits:
Animals
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Humans
Language:
En
Journal:
Hybridoma (Larchmt)
Year:
2012
Document type:
Article