QuaNCAT: quantitating proteome dynamics in primary cells.
Nat Methods
; 10(4): 343-6, 2013 Apr.
Article
in En
| MEDLINE
| ID: mdl-23474466
ABSTRACT
Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS), quantitative noncanonical amino acid tagging (QuaNCAT) allowed us to monitor the early expression changes of >600 proteins in primary resting T cells subjected to activation stimuli.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
CD4-Positive T-Lymphocytes
/
Gene Expression Regulation
/
Proteomics
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Nat Methods
Year:
2013
Document type:
Article