Fermentanomics informed amino acid supplementation of an antibody producing mammalian cell culture.
Biotechnol Prog
; 29(3): 745-53, 2013.
Article
in En
| MEDLINE
| ID: mdl-23606649
ABSTRACT
Fermentanomics, or a global understanding of a culture state on the molecular level empowered by advanced techniques like NMR, was employed to show that a model hybridoma culture supplied with glutamine and glucose depletes aspartate, cysteine, methionine, tryptophan, and tyrosine during antibody production. Supplementation with these amino acids prevents depletion and improves culture performance. Furthermore, no significant changes were observed in the distribution of glycans attached to the IgG3 in cultures supplemented with specific amino acids, arguing that this strategy can be implemented without fear of impact on important product quality attributes. In summary, a targeted strategy of quantifying media components and designing a supplementation strategy can improve bioprocess cell cultures when enpowered by fermentanomics tools.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biotechnology
/
Amino Acids
/
Antibodies
Limits:
Animals
Language:
En
Journal:
Biotechnol Prog
Year:
2013
Document type:
Article