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Magnetic barcode assay for genetic detection of pathogens.
Liong, Monty; Hoang, Anh N; Chung, Jaehoon; Gural, Nil; Ford, Christopher B; Min, Changwook; Shah, Rupal R; Ahmad, Rushdy; Fernandez-Suarez, Marta; Fortune, Sarah M; Toner, Mehmet; Lee, Hakho; Weissleder, Ralph.
Affiliation
  • Liong M; Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA.
Nat Commun ; 4: 1752, 2013.
Article in En | MEDLINE | ID: mdl-23612293
The task of rapidly identifying patients infected with Mycobacterium tuberculosis in resource-constrained environments remains a challenge. A sensitive and robust platform that does not require bacterial isolation or culture is critical in making informed diagnostic and therapeutic decisions. Here we introduce a platform for the detection of nucleic acids based on a magnetic barcoding strategy. PCR-amplified mycobacterial genes are sequence-specifically captured on microspheres, labelled by magnetic nanoprobes and detected by nuclear magnetic resonance. All components are integrated into a single, small fluidic cartridge for streamlined on-chip operation. We use this platform to detect M. tuberculosis and identify drug-resistance strains from mechanically processed sputum samples within 2.5 h. The specificity of the assay is confirmed by detecting a panel of clinically relevant non-M. tuberculosis bacteria, and the clinical utility is demonstrated by the measurements in M. tuberculosis-positive patient specimens. Combined with portable systems, the magnetic barcode assay holds promise to become a sensitive, high-throughput and low-cost platform for point-of-care diagnostics.
Subject(s)

Full text: 1 Collection: 01-internacional Health context: 3_ND Database: MEDLINE Main subject: DNA Barcoding, Taxonomic / Magnetics / Mycobacterium tuberculosis Type of study: Diagnostic_studies / Prognostic_studies Limits: Humans Language: En Journal: Nat Commun Year: 2013 Document type: Article

Full text: 1 Collection: 01-internacional Health context: 3_ND Database: MEDLINE Main subject: DNA Barcoding, Taxonomic / Magnetics / Mycobacterium tuberculosis Type of study: Diagnostic_studies / Prognostic_studies Limits: Humans Language: En Journal: Nat Commun Year: 2013 Document type: Article