In cell scalaradial interactome profiling using a bio-orthogonal clickable probe.

Cassiano, C; Margarucci, L; Esposito, R; Riccio, R; Tosco, A; Casapullo, A; Monti, M C

Cassiano, C; Margarucci, L; Esposito, R; Riccio, R; Tosco, A; Casapullo, A; Monti, M C.

Affiliation

Cassiano C; Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084, Fisciano, Salerno, Italy. mcmonti@unisa.it casapullo@unisa.it.

Chem Commun (Camb) ; 50(45): 6043-5, 2014 Jun 07.

Article in En | MEDLINE | ID: mdl-24769547

ABSTRACT

ABSTRACT

A bio-orthogonal click-chemistry procedure was developed to allow the in cell interactome profiling of scalaradial, an anti-inflammatory marine natural product. The results were validated through the application of the classical in vitro chemical proteomics and several bio-physical methods; peroxiredoxins, 14-3-3 isoforms and proteasomes were recognized as main scalaradial targets.

Subject(s)

14-3-3 Proteins/metabolism; Anti-Inflammatory Agents/pharmacology; Click Chemistry/methods; Homosteroids/pharmacology; Peroxiredoxins/metabolism; Sesterterpenes/pharmacology; 14-3-3 Proteins/analysis; Azides/chemistry; HeLa Cells; Homosteroids/chemistry; Humans; Peroxiredoxins/analysis; Sesterterpenes/chemistry; Tandem Mass Spectrometry

Fulltext

XML

PubMed Links

Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: 14-3-3 Proteins / Sesterterpenes / Peroxiredoxins / Click Chemistry / Homosteroids / Anti-Inflammatory Agents Limits: Humans Language: En Journal: Chem Commun (Camb) Year: 2014 Document type: Article

Fulltext

XML

PubMed Links

Search on Google