Perfusion decellularization of whole organs.

Guyette, Jacques P; Gilpin, Sarah E; Charest, Jonathan M; Tapias, Luis F; Ren, Xi; Ott, Harald C

Guyette, Jacques P; Gilpin, Sarah E; Charest, Jonathan M; Tapias, Luis F; Ren, Xi; Ott, Harald C.

Affiliation

Guyette JP; 1] Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA. [2] Harvard Medical School, Boston, Massachusetts, USA. [3].
Gilpin SE; 1] Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA. [2] Harvard Medical School, Boston, Massachusetts, USA. [3].
Charest JM; Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA.
Tapias LF; 1] Harvard Medical School, Boston, Massachusetts, USA. [2] Department of Surgery, Division of Thoracic Surgery, Massachusetts General Hospital, Boston, Massachusetts, USA.
Ren X; 1] Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA. [2] Harvard Medical School, Boston, Massachusetts, USA.
Ott HC; 1] Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA. [2] Harvard Medical School, Boston, Massachusetts, USA. [3] Department of Surgery, Division of Thoracic Surgery, Massachusetts General Hospital, Boston, Massachusetts, USA. [4] Harvard Stem Cell Institut

Nat Protoc ; 9(6): 1451-68, 2014.

Article in En | MEDLINE | ID: mdl-24874812

Subject(s)

Extracellular Matrix Proteins/isolation & purification; Extracellular Matrix/physiology; Perfusion/methods; Pressure; Tissue Engineering/methods; Viscera/cytology; Animals; Detergents; Humans; Rats; Swine; Tissue Scaffolds

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Perfusion / Pressure / Viscera / Extracellular Matrix Proteins / Tissue Engineering / Extracellular Matrix Limits: Animals / Humans Language: En Journal: Nat Protoc Year: 2014 Document type: Article

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