Western analysis of intracellular interleukin-8 in human mononuclear leukocytes.
Methods Mol Biol
; 1172: 285-93, 2014.
Article
in En
| MEDLINE
| ID: mdl-24908315
ABSTRACT
Most cytokines are stored in the cytoplasm until their release into the extracellular environment; however, some cytokines have been reported to localize in the nucleus. Traditional whole cell extract preparation does not provide information about the intracellular localization of cytokines. Here, we describe how to prepare cytoplasmic and nuclear extracts that can be analyzed by immunoblotting. While in this chapter we use this method to analyze intracellular localization of interleukin-8 (IL-8) in human mononuclear leukocytes, this protocol is adaptable to any cell type or protein of interest.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Leukocytes, Mononuclear
/
Cell Nucleus
/
Interleukin-8
/
Cytoplasm
Limits:
Humans
Language:
En
Journal:
Methods Mol Biol
Year:
2014
Document type:
Article