A sensitive, reproducible and objective immunofluorescence analysis method of dystrophin in individual fibers in samples from patients with duchenne muscular dystrophy.

Beekman, Chantal; Sipkens, Jessica A; Testerink, Janwillem; Giannakopoulos, Stavros; Kreuger, Dyonne; van Deutekom, Judith C; Campion, Giles V; de Kimpe, Sjef J; Lourbakos, Afrodite

Beekman, Chantal; Sipkens, Jessica A; Testerink, Janwillem; Giannakopoulos, Stavros; Kreuger, Dyonne; van Deutekom, Judith C; Campion, Giles V; de Kimpe, Sjef J; Lourbakos, Afrodite.

Affiliation

Beekman C; Prosensa Therapeutics BV, Leiden, the Netherlands.
Sipkens JA; Prosensa Therapeutics BV, Leiden, the Netherlands.
Testerink J; Prosensa Therapeutics BV, Leiden, the Netherlands.
Giannakopoulos S; Prosensa Therapeutics BV, Leiden, the Netherlands.
Kreuger D; Prosensa Therapeutics BV, Leiden, the Netherlands.
van Deutekom JC; Prosensa Therapeutics BV, Leiden, the Netherlands.
Campion GV; Prosensa Therapeutics BV, Leiden, the Netherlands.
de Kimpe SJ; Prosensa Therapeutics BV, Leiden, the Netherlands.
Lourbakos A; Prosensa Therapeutics BV, Leiden, the Netherlands.

PLoS One ; 9(9): e107494, 2014.

Article in En | MEDLINE | ID: mdl-25244123

Subject(s)

Dystrophin/metabolism; Fluorescent Antibody Technique/methods; Muscle Fibers, Skeletal/metabolism; Muscular Dystrophy, Duchenne/metabolism; Biopsy; Humans; Muscle Fibers, Skeletal/pathology; Muscular Dystrophy, Duchenne/pathology; Reproducibility of Results; Sensitivity and Specificity

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Dystrophin / Fluorescent Antibody Technique / Muscle Fibers, Skeletal / Muscular Dystrophy, Duchenne Type of study: Diagnostic_studies Limits: Humans Language: En Journal: PLoS One Year: 2014 Document type: Article

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