Autofluorescence analysis of dermatitis and squamous cell carcinoma in paraffin wax-embedded skin samples.
Clin Exp Dermatol
; 40(2): 123-8, 2015 Mar.
Article
in En
| MEDLINE
| ID: mdl-25251187
ABSTRACT
BACKGROUND:
Recent research has investigated the use of autofluorescence (AF) for distinguishing between normal and cancerous tissues according to different fluorescence characteristics.AIM:
To analyze if AF can help differentiate cancerous lesions from other nonneoplastic lesions, such as dermatitis, in each layer of the skin ex vivo.METHODS:
Paraffin wax-embedded tissue samples were obtained from patients who were histopathologically diagnosed with squamous cell carcinoma (SCC), psoriasis, chronic dermatitis (lichen simplex chronicus, prurigo nodularis) or acute dermatitis (atopic dermatitis). AF intensity was measured in four layers of the epidermis (corneal, granular, spinous and basal) and two layers of the dermis (papillary and reticular).RESULTS:
AF was highest in all layers of psoriasis samples compared with all layers of all other groups. Higher AF values were seen in SCC compared with all skin layers of acute and chronic dermatitis; this finding was especially true in the corneal layer, papillary dermis and reticular dermis.CONCLUSIONS:
This ex vivo AF study provides basic data for future in vivo studies of AF as a noninvasive diagnostic tool.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Psoriasis
/
Skin Neoplasms
/
Carcinoma, Squamous Cell
/
Dermatitis
/
Optical Imaging
Type of study:
Diagnostic_studies
/
Observational_studies
Limits:
Humans
Language:
En
Journal:
Clin Exp Dermatol
Year:
2015
Document type:
Article