Quantitative FRET measurement using emission-spectral unmixing with independent excitation crosstalk correction.

Zhang, J; Li, H; Chai, L; Zhang, L; Qu, J; Chen, T

Zhang, J; Li, H; Chai, L; Zhang, L; Qu, J; Chen, T.

Affiliation

Zhang J; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, China.

J Microsc ; 257(2): 104-16, 2015 Feb.

Article in En | MEDLINE | ID: mdl-25354559

Subject(s)

Fluorescence Resonance Energy Transfer/methods; Microscopy, Fluorescence/methods; Cell Line; Fluorescence Resonance Energy Transfer/standards; Humans; Luminescent Proteins/analysis; Microscopy, Fluorescence/standards

Key words

Crosstalk; FRET; emission-spectral unmixing; fluorescent proteins; living cells; microscopy

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Fluorescence Resonance Energy Transfer / Microscopy, Fluorescence Limits: Humans Language: En Journal: J Microsc Year: 2015 Document type: Article

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