Production of bispecific antibodies in "knobs-into-holes" using a cell-free expression system.

Xu, Yiren; Lee, John; Tran, Cuong; Heibeck, Tyler H; Wang, Willie D; Yang, Junhao; Stafford, Ryan L; Steiner, Alexander R; Sato, Aaron K; Hallam, Trevor J; Yin, Gang

Xu, Yiren; Lee, John; Tran, Cuong; Heibeck, Tyler H; Wang, Willie D; Yang, Junhao; Stafford, Ryan L; Steiner, Alexander R; Sato, Aaron K; Hallam, Trevor J; Yin, Gang.

Affiliation

Xu Y; a Sutro Biopharma, Inc. ; South San Francisco , CA USA.

MAbs ; 7(1): 231-42, 2015.

Article in En | MEDLINE | ID: mdl-25427258

Subject(s)

Antibodies, Bispecific/biosynthesis; Gene Expression; Single-Chain Antibodies/biosynthesis; Animals; Antibodies, Bispecific/genetics; CHO Cells; Cell-Free System/metabolism; Cricetinae; Cricetulus; Humans; Recombinant Proteins/biosynthesis; Recombinant Proteins/genetics; Single-Chain Antibodies/genetics

Key words

BiTE, bispecific T-cell engager; BiTE-KIH; CHO, Chinese hamster ovary; ELISA, enzyme-linked immunosorbent assay; EpCAM, epithelial cell adhesion molecule; FACS, fluorescence-activated cell sorting; Fab, antigen-binding fragment; Fc, fragment crystallizable; FcR, Fc receptor; HC, immunoglobulin heavy chain; HER2, human epidermal growth factor receptor 2; IgG, immunoglobulin G; KIH, knob-into-hole; LC, immunoglobulin light chain; LC-MS, liquid chromatography-mass spectrometry; PK, pharmacokinetics; bispecific antibody; cell-free protein expression; knob-into-hole; prefabrication; scFv, single-chain fragment variable; scFv-KIH

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Gene Expression / Antibodies, Bispecific / Single-Chain Antibodies Limits: Animals / Humans Language: En Journal: MAbs Year: 2015 Document type: Article

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