Thioredoxin rod-derived cone viability factor protects against photooxidative retinal damage.
Free Radic Biol Med
; 81: 22-9, 2015 Apr.
Article
in En
| MEDLINE
| ID: mdl-25596499
ABSTRACT
Rod-derived cone viability factor (RdCVF) is a trophic factor of the thioredoxins family that promotes the survival of cone photoreceptors. It is encoded by the nucleoredoxin-like gene 1 Nxnl1 which also encodes by alternative splicing a long form of RdCVF (RdCVFL), a thioredoxin enzyme that interacts with TAU. The known role of thioredoxins in the defense mechanism against oxidative damage led us to examine the retinal phenotype of the Nxnl1(-/-) mice exposed to photooxidative stress. Here we found that, in contrast to wild-type mice, the rod photoreceptors of Nxnl1(-/-) mice are more sensitive to light after exposure to 1700 or 2500 lx. The delivery of RdCVF by AAV to mice deficient of Nxnl1(-/-) protects rod photoreceptors from light damage. Interestingly, the RdCVF2L protein, encoded by the paralog gene Nxnl2, is able to reduce TAU phosphorylation, as does RdCVFL, but does not protect the rod from light damage. Our result shows that the Nxnl1 gene, through the thioredoxin RdCVFL, is part of an endogenous defense mechanism against photooxidative stress that is likely of great importance for human vision.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Thioredoxins
/
Genetic Therapy
/
Retinitis Pigmentosa
/
Retinal Rod Photoreceptor Cells
/
Retinal Cone Photoreceptor Cells
/
Eye Proteins
Type of study:
Etiology_studies
Limits:
Animals
Language:
En
Journal:
Free Radic Biol Med
Year:
2015
Document type:
Article