[Expressions of transient receptor potential A1 and related inflammatory factors in the rat model of prostatic inflammation].
Zhonghua Nan Ke Xue
; 21(1): 23-30, 2015 Jan.
Article
in Zh
| MEDLINE
| ID: mdl-25707135
ABSTRACT
OBJECTIVE:
To explore the molecular mechanism of pain associated with chronic prostatitis and chronic pelvic pain syndrome (CP/CPPS) in the rat model of prostatic inflammation.METHODS:
Thirty-six male SD rats were equally randomized to an experimental and a control group, the former injected with 50 µl of 3% λ-carrageenan into the ventral prostate to make the model of non-bacterial prostatic inflammation, while the latter with the same volume of sterile saline solution. At 1, 2 and 4 weeks after modeling, the prostate, L6-S1 dorsal root ganglion (DRG) and spinal cord were harvested for examination of the expressions of the nerve growth factor (NGF), transient receptor potential ankyrin 1 (TRPA1), and calcitonin-gene-related peptide (CGRP) by immunohistochemistry and Western blot.RESULTS:
The expressions of NGF, TRPA1 and CGRP in the prostatic tissue were all significantly increased in the experimental group as compared with the control (P <0.05), with a gradual decrease with the prolonging of time (P <0.05). In the L6-S1 DRG and spinal cord, the expressions of NGF, TRPA1 and CGRP exhibited no significant differences between the experimental and control groups at 1 week after modeling (P >0.05) and kept at high levels in the experimental group at 2 and 4 weeks, though not significantly different from those at 1 week (P >0.05). Statistically significant differences were observed in the expressions of the three proteins in the experimental rats among different time points (P <0.05), but not between the two groups at any time point (P >0.05).CONCLUSION:
The molecular mechanism of CP/CPPS can be evaluated in the rat model of prostatic inflammation established by injecting λ-carrageenan into the prostate. TRPA1 may play an important role in connecting the upstream and down-stream pathways of CP/CPPS-associated pain.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Prostatitis
/
Pelvic Pain
/
TRPC Cation Channels
/
Chronic Pain
Type of study:
Clinical_trials
/
Prognostic_studies
Limits:
Animals
/
Humans
/
Male
Language:
Zh
Journal:
Zhonghua Nan Ke Xue
Year:
2015
Document type:
Article