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Single-molecule microscopy of molecules tagged with GFP or RFP derivatives in mammalian cells using nanobody binders.
Platonova, Evgenia; Winterflood, Christian M; Junemann, Alexander; Albrecht, David; Faix, Jan; Ewers, Helge.
Affiliation
  • Platonova E; Randall Division of Cell and Molecular Biophysics, King's College London, SE1 1UL, United Kingdom; Institute of Biochemistry, ETH Zurich, Zurich, Switzerland.
  • Winterflood CM; Randall Division of Cell and Molecular Biophysics, King's College London, SE1 1UL, United Kingdom; Institute of Biochemistry, ETH Zurich, Zurich, Switzerland.
  • Junemann A; Hannover Medical School, Hannover, Germany.
  • Albrecht D; Randall Division of Cell and Molecular Biophysics, King's College London, SE1 1UL, United Kingdom; Institute of Biochemistry, ETH Zurich, Zurich, Switzerland.
  • Faix J; Hannover Medical School, Hannover, Germany.
  • Ewers H; Randall Division of Cell and Molecular Biophysics, King's College London, SE1 1UL, United Kingdom; Institute of Biochemistry, ETH Zurich, Zurich, Switzerland; Institut für Biochemie und Chemie, Freie Universität Berlin, Thielallee 63, 14195 Berlin, Germany. Electronic address: helge.ewers@fu-berlin.
Methods ; 88: 89-97, 2015 Oct 15.
Article in En | MEDLINE | ID: mdl-26123185

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Staining and Labeling / Fluorescent Antibody Technique / Green Fluorescent Proteins / Single-Domain Antibodies / Fluorescent Dyes / Luminescent Proteins / Microscopy, Fluorescence Limits: Animals / Humans Language: En Journal: Methods Year: 2015 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Staining and Labeling / Fluorescent Antibody Technique / Green Fluorescent Proteins / Single-Domain Antibodies / Fluorescent Dyes / Luminescent Proteins / Microscopy, Fluorescence Limits: Animals / Humans Language: En Journal: Methods Year: 2015 Document type: Article