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DNA RECOMBINATION. Base triplet stepping by the Rad51/RecA family of recombinases.
Lee, Ja Yil; Terakawa, Tsuyoshi; Qi, Zhi; Steinfeld, Justin B; Redding, Sy; Kwon, YoungHo; Gaines, William A; Zhao, Weixing; Sung, Patrick; Greene, Eric C.
Affiliation
  • Lee JY; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
  • Terakawa T; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA. Department of Biophysics, Kyoto University, Sakyo, Kyoto, Japan.
  • Qi Z; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
  • Steinfeld JB; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
  • Redding S; Department of Chemistry, Columbia University, New York, NY, USA.
  • Kwon Y; Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT, USA.
  • Gaines WA; Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT, USA.
  • Zhao W; Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT, USA.
  • Sung P; Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT, USA.
  • Greene EC; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA. Howard Hughes Medical Institute, Columbia University, New York, NY, USA. ecg2108@cumc.columbia.edu.
Science ; 349(6251): 977-81, 2015 Aug 28.
Article in En | MEDLINE | ID: mdl-26315438
ABSTRACT
DNA strand exchange plays a central role in genetic recombination across all kingdoms of life, but the physical basis for these reactions remains poorly defined. Using single-molecule imaging, we found that bacterial RecA and eukaryotic Rad51 and Dmc1 all stabilize strand exchange intermediates in precise three-nucleotide steps. Each step coincides with an energetic signature (0.3 kBT) that is conserved from bacteria to humans. Triplet recognition is strictly dependent on correct Watson-Crick pairing. Rad51, RecA, and Dmc1 can all step over mismatches, but only Dmc1 can stabilize mismatched triplets. This finding provides insight into why eukaryotes have evolved a meiosis-specific recombinase. We propose that canonical Watson-Crick base triplets serve as the fundamental unit of pairing interactions during DNA recombination.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rec A Recombinases / DNA / Saccharomyces cerevisiae Proteins / Recombinases / Rad51 Recombinase / Homologous Recombination Limits: Humans Language: En Journal: Science Year: 2015 Document type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rec A Recombinases / DNA / Saccharomyces cerevisiae Proteins / Recombinases / Rad51 Recombinase / Homologous Recombination Limits: Humans Language: En Journal: Science Year: 2015 Document type: Article