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Homologous pairing and the formation of nascent synaptic intermediates between regions of duplex DNA by RecA protein.
Conley, E C; West, S C.
Affiliation
  • Conley EC; Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, England.
Cell ; 56(6): 987-95, 1989 Mar 24.
Article in En | MEDLINE | ID: mdl-2647306
The RecA protein from E. coli gains access to duplex DNA, by nucleation from a short single-stranded gap, to form a spiral nucleoprotein filament that is capable of interaction with homologous duplex DNA. The observations described here demonstrate that any part of the nucleoprotein filament, whether it contains single- or double-stranded DNA, is capable of pairing with homologous duplex DNA. Homologous contacts between regions of duplex DNA lead to an increase in the initial rate and final extent of joint molecule formation. The experiments indicate that pairing is facilitated by the formation of nascent synaptic intermediates between duplex DNA sequences. Using chimeric form I DNA, which is incapable of forming an inter-wound or plectonemic joint with the gapped DNA due to the presence of flanking heterologous sequences, we show that these duplex-duplex pairing reactions involve extensive underwinding of the double helix.
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Collection: 01-internacional Health context: 3_ND Database: MEDLINE Main subject: Rec A Recombinases / Synaptonemal Complex / DNA / Base Sequence / Sequence Homology, Nucleic Acid / Meiosis Language: En Journal: Cell Year: 1989 Document type: Article
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Collection: 01-internacional Health context: 3_ND Database: MEDLINE Main subject: Rec A Recombinases / Synaptonemal Complex / DNA / Base Sequence / Sequence Homology, Nucleic Acid / Meiosis Language: En Journal: Cell Year: 1989 Document type: Article