Erythropoietin increases macrophage-mediated T cell suppression.
Cell Immunol
; 306-307: 17-24, 2016.
Article
in En
| MEDLINE
| ID: mdl-27262376
ABSTRACT
Erythropoietin (EPO), used to treat anemia in cancer patients, has been reported to accelerate tumor progression and increase mortality. Research of the mechanism for this effect has focused upon EPOR expression by tumor cells. We model the high macrophage to lymphocyte ratio found in tumor microenvironments (TMEs) by culturing peritoneal cavity (PerC) cells that naturally have a high macrophage to T cell ratio. Following TCR ligation, C57BL/6J PerC T cell proliferation is suppressed due to IFNγ-triggered inducible nitric oxide synthase (iNOS) expression. EPO was tested in the PerC culture model and found to increase T cell suppression. This effect could be abrogated by inhibiting iNOS by enzyme inhibition, genetic ablation, or blocking IFNγ signaling. Flow cytometry revealed the EPOR on CD11b(+)F4/80(+) macrophages. These results suggest that EPO could increase T cell suppression in the TME by acting directly on macrophages.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peritoneal Cavity
/
T-Lymphocytes
/
Erythropoietin
/
Receptors, Erythropoietin
/
Macrophages
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
En
Journal:
Cell Immunol
Year:
2016
Document type:
Article