Site selection by Xenopus laevis RNAase P.
Cell
; 58(1): 37-45, 1989 Jul 14.
Article
in En
| MEDLINE
| ID: mdl-2752421
ABSTRACT
Investigation of the mechanism of cleavage site selection by Xenopus RNAase P reveals that the acceptor stem, a 7 bp helix common to all tRNA precursors, is required for cleavage. We propose that Xenopus RNAase P recognizes conserved features of the mature tRNA and that the cleavage site is selected by measuring the length of the acceptor stem. In support of this, we demonstrate that insertion of 2 bp in the acceptor stem of yeast pre-tRNA(3Leu) relocates the cleavage site 2 bases 3' to the original one. In addition, insertion of 1 bp in the acceptor stem of the end-matured yeast pre-tRNA(Phe) generates an RNAase P cleavage site the enzyme produces a mature tRNA with the characteristic 7 bp stem and releases one 5' flanking nucleotide. Since it has previously been shown that cleavage sites of the splicing endonuclease are determined by the length of the anticodon stem, RNAase P and the splicing endonuclease apparently use different stems to determine their cutting sites.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Xenopus laevis
/
RNA, Transfer
/
Endoribonucleases
/
Nucleic Acid Precursors
Limits:
Animals
Language:
En
Journal:
Cell
Year:
1989
Document type:
Article