[Multilocus Sequence Typing analysis of human Campylobacter coli in Granada (Spain)].
Rev Esp Quimioter
; 29(6): 332-335, 2016 12.
Article
in En
| MEDLINE
| ID: mdl-27701619
ABSTRACT
OBJECTIVE:
Different subtypes of Campylobacter spp. have been associated with diarrhoea and a Multilocus Sequence Typing (MLST) method has been performed for subtyping. In the present work, MLST was used to analyse the genetic diversity of eight strains of Campylobacter coli.METHODS:
Nineteen genetic markers were amplified for MLSTanalysis:
AnsB, DmsA, ggt, Cj1585c, CJJ81176-1367/1371, Tlp7, cj1321-cj1326, fucP, cj0178, cj0755/cfrA, ceuE, pldA, cstII, cstIII. After comparing the obtained sequences with the Campylobacter MLST database, the allele numbers, sequence types (STs) and clonal complexes (CCs) were assigned.RESULTS:
The 8 C. coli isolates yielded 4 different STs belonging to 2 CCs. Seven isolates belong to ST-828 clonal complex and only one isolate belong to ST-21. Two samples came from the same patient, but were isolated in two different periods of time.CONCLUSIONS:
MLST can be useful for taxonomic characterization of C. coli isolates.
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Collection:
01-internacional
Health context:
3_ND
Database:
MEDLINE
Main subject:
Campylobacter coli
/
Multilocus Sequence Typing
Limits:
Adolescent
/
Adult
/
Child
/
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
Country/Region as subject:
Caribe ingles
/
Europa
/
Grenada
Language:
En
Journal:
Rev Esp Quimioter
Year:
2016
Document type:
Article