Development and evaluation of a rapid recombinase polymerase amplification assay for detection of coxsackievirus A6.
Arch Virol
; 162(1): 287-290, 2017 Jan.
Article
in En
| MEDLINE
| ID: mdl-27722991
ABSTRACT
Coxsackievirus A6 (CV-A6) is an important pathogen causing hand, foot and mouth disease (HFMD). The aim of this study was to develop and evaluate a rapid real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of CV-A6. The sensitivity of this assay was 202 copies/reaction, with 100 % specificity. Furthermore, this assay yielded consistent results comparable with a commercial qRT-PCR diagnostic kit. This assay is therefore potentially useful for surveillance of CV-A6 infections and outbreak control.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Enterovirus
/
Reverse Transcriptase Polymerase Chain Reaction
/
Nucleic Acid Amplification Techniques
/
Real-Time Polymerase Chain Reaction
/
Hand, Foot and Mouth Disease
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Child, preschool
/
Humans
/
Infant
/
Newborn
Language:
En
Journal:
Arch Virol
Year:
2017
Document type:
Article