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Development and evaluation of a rapid recombinase polymerase amplification assay for detection of coxsackievirus A6.
Wang, Kaifeng; Wu, Yue; Yin, Dan; Tang, Shixing; Hu, Guifang; He, Yaqing.
Affiliation
  • Wang K; Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, 510515, China.
  • Wu Y; Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, 510515, China.
  • Yin D; Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, 510515, China.
  • Tang S; Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, 510515, China.
  • Hu G; Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, 510515, China. guif_hu@sina.com.
  • He Y; Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, Guangdong, China. heyaqing1019@126.com.
Arch Virol ; 162(1): 287-290, 2017 Jan.
Article in En | MEDLINE | ID: mdl-27722991
ABSTRACT
Coxsackievirus A6 (CV-A6) is an important pathogen causing hand, foot and mouth disease (HFMD). The aim of this study was to develop and evaluate a rapid real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of CV-A6. The sensitivity of this assay was 202 copies/reaction, with 100 % specificity. Furthermore, this assay yielded consistent results comparable with a commercial qRT-PCR diagnostic kit. This assay is therefore potentially useful for surveillance of CV-A6 infections and outbreak control.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Enterovirus / Reverse Transcriptase Polymerase Chain Reaction / Nucleic Acid Amplification Techniques / Real-Time Polymerase Chain Reaction / Hand, Foot and Mouth Disease Type of study: Diagnostic_studies / Evaluation_studies Limits: Child, preschool / Humans / Infant / Newborn Language: En Journal: Arch Virol Year: 2017 Document type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Enterovirus / Reverse Transcriptase Polymerase Chain Reaction / Nucleic Acid Amplification Techniques / Real-Time Polymerase Chain Reaction / Hand, Foot and Mouth Disease Type of study: Diagnostic_studies / Evaluation_studies Limits: Child, preschool / Humans / Infant / Newborn Language: En Journal: Arch Virol Year: 2017 Document type: Article