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Development and validation of an UPLC-Q/TOF-MS assay for the quantitation of neopanaxadiol in beagle dog plasma: Application to a pharmacokinetic study.
Geng, Cong; Wang, Chun-Hong; Hu, Hong; Gao, Xiao-Ping; Gong, Ai-Hua; Lin, Ying-Wei; Fan, Xiu-Shuang; Li, Heng; Yin, Jian-Yuan.
Affiliation
  • Geng C; Department of Clinical Laboratory, The Second Affiliated Hospital of Dalian Medical University, 467 Zhongshan Road, Dalian, 116023, People's Republic of China.
  • Wang CH; Department of Natural Products Chemistry, College of Pharmacy, Jilin University, 1266 Fujin Road, Changchun, 130021, People's Republic of China.
  • Hu H; Department of Clinical Laboratory, The Second Affiliated Hospital of Dalian Medical University, 467 Zhongshan Road, Dalian, 116023, People's Republic of China.
  • Gao XP; Department of Clinical Laboratory, The Second Affiliated Hospital of Dalian Medical University, 467 Zhongshan Road, Dalian, 116023, People's Republic of China.
  • Gong AH; Department of Clinical Laboratory, The Second Affiliated Hospital of Dalian Medical University, 467 Zhongshan Road, Dalian, 116023, People's Republic of China.
  • Lin YW; Department of Clinical Laboratory, The Second Affiliated Hospital of Dalian Medical University, 467 Zhongshan Road, Dalian, 116023, People's Republic of China.
  • Fan XS; Department of Natural Products Chemistry, College of Pharmacy, Jilin University, 1266 Fujin Road, Changchun, 130021, People's Republic of China.
  • Li H; Department of Natural Products Chemistry, College of Pharmacy, Jilin University, 1266 Fujin Road, Changchun, 130021, People's Republic of China.
  • Yin JY; Department of Natural Products Chemistry, College of Pharmacy, Jilin University, 1266 Fujin Road, Changchun, 130021, People's Republic of China.
Biomed Chromatogr ; 31(5)2017 May.
Article in En | MEDLINE | ID: mdl-27790730
ABSTRACT
Neopanaxadiol (NPD), the main panaxadiol constituent of Panax ginseng C. A. Meyer (Araliaceae), has been regarded as the active component for the treatment of Alzheimer's disease. However, few references are available about pharmacokinetic evaluation for NPD. Accordingly, a rapid and sensitive method for quantitative analysis of NPD in beagle dog plasma based on ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry was developed and validated. Analytes were extracted from plasma by liquid-liquid extraction and chromatographic separation was achieved on an Agilent Zorbax Stable Bond C18 column. Detection was performed in the positive ion mode using multiple reaction monitoring of the transitions both at m/z 461.4 → 425.4 for NPD and internal standard of panaxadiol. All validation parameters, such as lower limit of quantitation, linearity, specificity, precision, accuracy, extraction recovery, matrix effect and stability, were within acceptable ranges and the method was appropriate for multitude sample determination. After oral intake, NPD was slowly absorbed and eliminated from circulatory blood system and corresponding plasma exposure was low. Application of this quantitative method will yield the first pharmacokinetic profile after oral administration of NPD to beagle dog. The information obtained here will be useful to understand the pharmacological effects of NPD.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Mass Spectrometry / Chromatography, High Pressure Liquid / Ginsenosides Limits: Animals Language: En Journal: Biomed Chromatogr Year: 2017 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Mass Spectrometry / Chromatography, High Pressure Liquid / Ginsenosides Limits: Animals Language: En Journal: Biomed Chromatogr Year: 2017 Document type: Article