Your browser doesn't support javascript.
loading
A conserved region in the Closterovirus 1a polyprotein drives extensive remodeling of endoplasmic reticulum membranes and induces motile globules in Nicotiana benthamiana cells.
Gushchin, V A; Karlin, D G; Makhotenko, A V; Khromov, A V; Erokhina, T N; Solovyev, A G; Morozov, S Yu; Agranovsky, A A.
Affiliation
  • Gushchin VA; Faculty of Biology, Moscow State University, Moscow 119991, Russia; N.F. Gamaleya Federal Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Russia.
  • Karlin DG; 25, rue de Cassis, 13008 Marseille, France.
  • Makhotenko AV; Faculty of Biology, Moscow State University, Moscow 119991, Russia.
  • Khromov AV; Faculty of Biology, Moscow State University, Moscow 119991, Russia.
  • Erokhina TN; M.M. Shemyakin and Y.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.
  • Solovyev AG; A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russia; Sechenov First Moscow State Medical University, Institute of Molecular Medicine, Moscow 119991, Russia.
  • Morozov SY; A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russia.
  • Agranovsky AA; Faculty of Biology, Moscow State University, Moscow 119991, Russia; Center of Bioengineering, Russian Academy of Sciences, Moscow, Russia. Electronic address: aaa@genebee.msu.su.
Virology ; 502: 106-113, 2017 02.
Article in En | MEDLINE | ID: mdl-28027478
In infected plant cells, closterovirus replicative polyproteins 1a and 1ab drive membrane remodeling and formation of multivesicular replication platforms. Polyprotein 1a contains a variable Central Region (CR) between the methyltransferase and helicase domains. In a previous study, we have found that transient expression of the Beet yellows virus CR-2 segment (aa 1305-1494) in Nicotiana benthamiana induces the formation of ~1µm mobile globules originating from the ER membranes. In the present study, sequence analysis has shown that a part of the CR named the "Zemlya region" (overlapping the CR-2), is conserved in all members of the Closterovirus genus and contains a predicted amphipathic helix (aa 1368-1385). By deletion analysis, the CR-2 region responsible for the induction of 1-µm globules has been mapped to aa 1368-1432. We suggest that the conserved membrane-modifying region of the BYV 1a may be involved in the biogenesis of closterovirus replication platforms.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Diseases / Nicotiana / Viral Proteins / Closterovirus / Polyproteins / Endoplasmic Reticulum Type of study: Prognostic_studies Language: En Journal: Virology Year: 2017 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Diseases / Nicotiana / Viral Proteins / Closterovirus / Polyproteins / Endoplasmic Reticulum Type of study: Prognostic_studies Language: En Journal: Virology Year: 2017 Document type: Article