The antioxidative and hepatoprotective effects comparison of Chinese angelica polysaccharide(CAP)and selenizing CAP (sCAP) in CCl4 induced hepatic injury mice.
Int J Biol Macromol
; 97: 46-54, 2017 Apr.
Article
in En
| MEDLINE
| ID: mdl-28064055
ABSTRACT
Chinese angelica polysaccharides (CAP) and selenizing CAP (sCAP) were prepared and identified through FTIR and SEM observation. Their antioxidant activities in vitro and hepatoprotective effects in vivo were compared by free radical-scavenging tests or with CCl4-induced hepatic injury model mice. The results showed that for DPPH radical, superoxide anion and hydroxyl radical, the scavenging capabilities of sCAP were significantly stronger than those of CAP. In hepatic injury model mice, sCAP could significantly reduce ALT, AST and ALP contents and raised TP content in serum, significantly reduce MDA and ROS contents and raised SOD and T-AOC activities in liver homogenate in comparison with CAP; obviously relieve the pathological changes of liver and significantly inhibit the expressions of p-ERK, p-JNK and p-p38 protein as compared with those in model control group. These results indicate that selenylation modification can enhance the antioxidant and hepatoprotective actions of Chinese angelica polysaccharide. A action mechanism of sCAP is suppressing the protein expression of MAPK signaling pathway.
Key words
1,10-phenanthroline (PubChem CID: 84567); 2,2-Diphenyl-1-picrylhydrazyl (PubChem CID: 74358); Antioxidative activity; Carbon tetrachloride (PubChem CID: 5943); Chinese angelica polysaccharides; Ferrous sulfate (PubChem CID: 24393); Hepatoprotective activity; Hydrochloric acid (PubChem CID: 313); Hydrogen peroxide (PubChem CID: 784); MAPK signaling pathway; Nitric acid (PubChem CID: 944); Oxidative stress; Potassium bromide (PubChem CID: 253877); Selenizing chinese angelica polysaccharides; Sodium selenite (PubChem CID: 24934); Tris (PubChem CID: 6503)
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polysaccharides
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Selenium
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Carbon Tetrachloride
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Angelica sinensis
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Chemical and Drug Induced Liver Injury
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Liver
Limits:
Animals
Language:
En
Journal:
Int J Biol Macromol
Year:
2017
Document type:
Article