Honeybee locomotion is impaired by Am-CaV3 low voltage-activated Ca2+ channel antagonist.
Sci Rep
; 7: 41782, 2017 02 01.
Article
in En
| MEDLINE
| ID: mdl-28145504
ABSTRACT
Voltage-gated Ca2+ channels are key transducers of cellular excitability and participate in several crucial physiological responses. In vertebrates, 10 Ca2+ channel genes, grouped in 3 families (CaV1, CaV2 and CaV3), have been described and characterized. Insects possess only one member of each family. These genes have been isolated in a limited number of species and very few have been characterized although, in addition to their crucial role, they may represent a collateral target for neurotoxic insecticides. We have isolated the 3 genes coding for the 3 Ca2+ channels expressed in Apis mellifera. This work provides the first detailed characterization of the honeybee T-type CaV3 Ca2+ channel and demonstrates the low toxicity of inhibiting this channel. Comparing Ca2+ currents recorded in bee neurons and myocytes with Ca2+ currents recorded in Xenopus oocytes expressing the honeybee CaV3 gene suggests native expression in bee muscle cells only. High-voltage activated Ca2+ channels could be recorded in the somata of different cultured bee neurons. These functional data were confirmed by in situ hybridization, immunolocalization and in vivo analysis of the effects of a CaV3 inhibitor. The biophysical and pharmacological characterization and the tissue distribution of CaV3 suggest a role in honeybee muscle function.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bees
/
Calcium Channel Blockers
/
Calcium Channels, T-Type
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Locomotion
Limits:
Animals
Language:
En
Journal:
Sci Rep
Year:
2017
Document type:
Article