[Mendelian randomization analysis of the relationship between obesity and DNA methylation].
Zhonghua Yu Fang Yi Xue Za Zhi
; 51(2): 137-142, 2017 Feb 06.
Article
in Zh
| MEDLINE
| ID: mdl-28219152
ABSTRACT
Objective:
To explore the association between DNA methylation and body mass index (BMI) using Mendelian randomization analysis.Methods:
A total of 469 participants were selected from the Chinese National Twin Registry in 2013, who were living in Shandong, Jiangsu, Zhejiang, and Sichuan provinces, and at least 18 years of age. A questionnaire survey and physical examination were conducted to collect demographic, clinical, and behavioral information. Peripheral blood cells were collected to detect genotype and methylation status. Association analyses between DNA methylation and BMI and between CpGs and cis-SNP were conducted. With rs748212 as the instrumental variable, the association between cg15053022 and BMI was explored using the Mendelian randomization method.Results:
A total of 469 participants were selected. The mean age of participants was (44.8±13.2) years and the BMI was (25.0±3.8) kg/m(2). Nine BMI-related DNA methylation sites were found and DNA methylation site cg15053022 in the ATP4A gene was negatively associated with cis-SNP rs748212 (ß=-0.020); the mean methylation level of AA, AC, and CC were 0.212±0.025, 0.242±0.024, and 0.264±0.028, respectively. rs748212 was associated with BMI (ß=0.04, P=0.007) and closely related to cg15053022 (F=237.66, P=0.143). Mendelian randomization analysis showed lower methylation levels at cg15053022 were associated with higher BMI (ß=-1.97, P<0.001).Conclusion:
This study supported the impact of cg15053022 methylation in the ATP4A gene on BMI using Mendelian randomization analysis and provided the basis for using Mendelian randomization analysis in methylation studies.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA Methylation
/
Asian People
/
Mendelian Randomization Analysis
/
Obesity
Type of study:
Clinical_trials
Limits:
Adult
/
Humans
/
Middle aged
Language:
Zh
Journal:
Zhonghua Yu Fang Yi Xue Za Zhi
Year:
2017
Document type:
Article