Immunization of C57BL/6 Mice with GRA2 Combined with MPL Conferred Partial Immune Protection against Toxoplasma gondii
Iran Biomed J
; 22(1): 22-32, 2018 01 01.
Article
in En
| MEDLINE
| ID: mdl-28646827
ABSTRACT
Background:
We have previously reported that immunization with GRA2 antigen of Toxoplasma gondii induces protective immunity in CBA/J (H2k) and BALB/c mice (H2d). We aimed to examine whether immunization of a distinct strain of rodent with recombinant dense granule antigens (GRA2) combined with monophosphorryl lipid A (MPL) adjuvant elicits protective immune response against T. gondii.Methods:
C57BL/6 (H2b haplotype) mice were immunized with GRA2, formulated in MPL adjuvant.Results:
Strong humoral response, predominantly of IgG1 subclass and cellular response, IFN-γ, was detected at three weeks post immunization. Mice immunized with GRA2 had significantly (p < 0.01) fewer brain cysts than those in the adjuvant group, upon challenge infection. Despite the production of a strong antibody response, IFN-γ production and brain cyst reduction were not significant when the immunized mice were infected four months after the immunization.Conclusion:
We can conclude that GRA2 immunization partially protects against T. gondii infection in C57BL/6 mice, though the potency and longevity of this antigen as a standalone vaccine may vary in distinct genetic backgrounds. This observation further emphasizes the utility of GRA2 for incorporation into a multi-antigenic vaccine against T. gondii.
Full text:
1
Collection:
01-internacional
Health context:
3_ND
Database:
MEDLINE
Language:
En
Journal:
Iran Biomed J
Year:
2018
Document type:
Article