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Profiling Abscisic Acid-Induced Changes in Fatty Acid Composition in Mosses.
Shinde, Suhas; Devaiah, Shivakumar; Kilaru, Aruna.
Affiliation
  • Shinde S; Department of Biological Sciences, East Tennessee State University, Johnson City, TN, USA.
  • Devaiah S; Department of Biological Sciences, East Tennessee State University, Johnson City, TN, USA.
  • Kilaru A; Department of Biological Sciences, East Tennessee State University, Johnson City, TN, USA. kilaru@etsu.edu.
Methods Mol Biol ; 1631: 295-303, 2017.
Article in En | MEDLINE | ID: mdl-28735405
ABSTRACT
In plants, change in lipid composition is a common response to various abiotic stresses. Lipid constituents of bryophytes are of particular interest as they differ from that of flowering plants. Unlike higher plants, mosses have high content of very long-chain polyunsaturated fatty acids. Such lipids are considered to be important for survival of nonvascular plants. Here, using abscisic acid (ABA )-induced changes in lipid composition in Physcomitrella patens as an example, a protocol for total lipid extraction and quantification by gas chromatography (GC) coupled with flame ionization detector (FID) is described.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bryopsida / Fatty Acids Limits: Animals Language: En Journal: Methods Mol Biol Year: 2017 Document type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bryopsida / Fatty Acids Limits: Animals Language: En Journal: Methods Mol Biol Year: 2017 Document type: Article