[The effect of aluminum trichloride on expression of phosphorylated tau and Aß in SH-SY5Y cells].

ABSTRACT

Objective: To investigate the expression of phosphorylated tau (p-tau) and Aß in SH-SY5Y cells induced by aluminum or/and ApoE ε4 allele, and study the interaction between aluminum and ApoE ε4 allele. Methods: SH-SY5Y cells were assigned to control group, 400 µmol/L AlCl(3) group, ApoEε4 transfected group and 400 µmol/L AlCl(3) with Apo Eε4 transfected group. The cell viability was measured by CCK-8 assay; the expressions of p-tau and Aß was determined with ELISA Kit after AlCl(3) exposure or or/and ApoE ε4 transfection. Results: The viability of cells exposed to 400 µmol/L AlCl(3) or/and ApoE ε4 transfected were significantly lower than that of controls (P<0.05) . The expressions of total tau, tau-181, tau-231, tau-396 and Aß in 400 µmol/L Al(3)+ or/and ApoE ε4 transfected exposed cells showed significantly higher than those of controls (P<0.05) . Based on the factorial design, a significant interaction exists, and there is a synergistic effect between AlCl(3) and ApoE ε4 (P<0.05) . Conclusion: Aluminum and ApoE ε4 allele could increase expression of p-tau and Aß deposition; there was a synergistic interaction between aluminum and ApoE ε4 allele on cell death, tau phosphorylation and Aß deposition of SH-SY5Y.