The CTX-M-14 plasmid pHK01 encodes novel small RNAs and influences host growth and motility.
FEMS Microbiol Ecol
; 93(7)2017 07 01.
Article
in En
| MEDLINE
| ID: mdl-28854680
ABSTRACT
The dissemination of extended-spectrum ß-lactamases (ESBLs) genes among bacteria is commonly achieved by plasmid conjugation. In the last decade, the CTX-M type enzyme was the most widespread and prevalent ESBLs in the world. In Hong Kong and mainland China, among the commonly found CTX-M-carrying plasmids were pHK01 and pHK01-like plasmids, which belong to incompatibility group FII (IncFII). In this work, we studied the physiological effect caused by the pHK01 plasmid in bacterial host Escherichia coli J53. The plasmid did not affect cell growth of the host but reduced their motility. The reduction of host motility was attributed to downregulation of genes that encode the flagellar system. We also identified several plasmid-encoded sRNAs, and showed that the overexpression of one of them, AS-traI, in the presence of pHK01 plasmid shortened the lag phase of host growth. In addition to the study of pHK01 in bacteria, we also developed a fast and incompatibility group-specific curing method using countertranscribed RNA, which could be of general usage for studying plasmid-host interaction in clinical aspects.
Key words
Full text:
1
Collection:
01-internacional
Health context:
2_ODS3
/
3_ND
Database:
MEDLINE
Main subject:
Plasmids
/
Beta-Lactamases
/
RNA, Bacterial
/
Drug Resistance, Multiple, Bacterial
/
Escherichia coli
Limits:
Humans
Country/Region as subject:
Asia
Language:
En
Journal:
FEMS Microbiol Ecol
Year:
2017
Document type:
Article