Generation and CRISPR/Cas9 editing of transformed progenitor B cells as a pseudo-physiological system to study DNA repair gene function in V(D)J recombination.

Lenden Hasse, Hélène; Lescale, Chloé; Bianchi, Joy J; Yu, Wei; Bedora-Faure, Marie; Deriano, Ludovic

Lenden Hasse, Hélène; Lescale, Chloé; Bianchi, Joy J; Yu, Wei; Bedora-Faure, Marie; Deriano, Ludovic.

Affiliation

Lenden Hasse H; Genome Integrity, Immunity and Cancer Unit, Department of Immunology, Department of Genomes and Genetics, Institut Pasteur, 75015 Paris, France.
Lescale C; Genome Integrity, Immunity and Cancer Unit, Department of Immunology, Department of Genomes and Genetics, Institut Pasteur, 75015 Paris, France.
Bianchi JJ; Genome Integrity, Immunity and Cancer Unit, Department of Immunology, Department of Genomes and Genetics, Institut Pasteur, 75015 Paris, France; Cellule Pasteur, University of Paris René Descartes, Sorbonne Paris Cité, Paris 75015, France.
Yu W; Genome Integrity, Immunity and Cancer Unit, Department of Immunology, Department of Genomes and Genetics, Institut Pasteur, 75015 Paris, France.
Bedora-Faure M; Genome Integrity, Immunity and Cancer Unit, Department of Immunology, Department of Genomes and Genetics, Institut Pasteur, 75015 Paris, France.
Deriano L; Genome Integrity, Immunity and Cancer Unit, Department of Immunology, Department of Genomes and Genetics, Institut Pasteur, 75015 Paris, France. Electronic address: ludovic.deriano@pasteur.fr.

J Immunol Methods ; 451: 71-77, 2017 12.

Article in En | MEDLINE | ID: mdl-28882611

Subject(s)

CRISPR-Associated Proteins/genetics; CRISPR-Cas Systems; Clustered Regularly Interspaced Short Palindromic Repeats; DNA Breaks, Double-Stranded; Gene Editing/methods; Precursor Cells, B-Lymphoid/metabolism; Recombinational DNA Repair; Animals; Apoptosis/drug effects; CRISPR-Associated Proteins/metabolism; Cell Line, Transformed; DNA End-Joining Repair; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; G1 Phase Cell Cycle Checkpoints/drug effects; Genotype; Homeodomain Proteins/genetics; Homeodomain Proteins/metabolism; Imatinib Mesylate/pharmacology; Mice, Inbred C57BL; Oncogene Proteins v-abl/antagonists & inhibitors; Oncogene Proteins v-abl/genetics; Oncogene Proteins v-abl/metabolism; Phenotype; Precursor Cells, B-Lymphoid/drug effects; Precursor Cells, B-Lymphoid/pathology; Protein Kinase Inhibitors/pharmacology; Proto-Oncogene Proteins c-bcl-2/genetics; Proto-Oncogene Proteins c-bcl-2/metabolism; Recombinational DNA Repair/drug effects

Key words

CRISPR/Cas9-mediated gene knock-out; Nonhomologous end joining (NHEJ); Recombination-activating gene (RAG) endonuclease; V(D)J recombination; v-Abl transformed pro-B cells

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA Breaks, Double-Stranded / Precursor Cells, B-Lymphoid / Recombinational DNA Repair / CRISPR-Associated Proteins / Clustered Regularly Interspaced Short Palindromic Repeats / CRISPR-Cas Systems / Gene Editing Type of study: Prognostic_studies Limits: Animals Language: En Journal: J Immunol Methods Year: 2017 Document type: Article

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