HMG-box factor SoxD/Sox15 and homeodomain-containing factor Xanf1/Hesx1 directly interact and regulate the expression of Xanf1/Hesx1 during early forebrain development in Xenopus laevis.
Gene
; 638: 52-59, 2018 Jan 05.
Article
in En
| MEDLINE
| ID: mdl-28918251
ABSTRACT
The homeodomain-containing transcription factor Anf (also known as Rpx/Hesx1 in mammals) plays an important role during the forebrain development in vertebrates. Here we demonstrate the ability of the Xenopus laevis Anf, Xanf1/Hesx1, to directly bind SRY-related HMG-box-containing transcription factor SoxD/Sox15 and to cooperate with the latter during regulating of the expression of Xanf1/Hesx1 own gene. As we have shown by GST pull-down, EMSA and the luciferase reporter assays, Xanf1/Hesx1 and SoxD/Sox15 bind the Xanf1/Hesx1 promoter region counteracting the inhibitory effect of Xanf1/Hesx1 alone. This finding explains how Xanf1/Hesx1 could escape the repressive activity of its own protein during early patterning of the forebrain rudiment.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Xenopus laevis
/
Prosencephalon
/
Homeodomain Proteins
/
Xenopus Proteins
/
SOXD Transcription Factors
Limits:
Animals
Language:
En
Journal:
Gene
Year:
2018
Document type:
Article