MicroRNA242 is associated with cell proliferation, invasion, migration and apoptosis in renal cell carcinoma.
Mol Med Rep
; 16(6): 9157-9164, 2017 Dec.
Article
in En
| MEDLINE
| ID: mdl-28990105
ABSTRACT
Micro (mi)RNAs are involved in multiple cellular processes, and alterations in miRNA expression have been demonstrated to lead to tumorigenesis. Previous microarray analysis revealed that miRNA (miR)24 was downregulated in renal cell carcinoma (RCC). Additionally, miR24 has been identified as an oncogene and tumor suppressor in various cancers. The present study assessed the expression levels of two stemloops of miR24, miR241 and miR242, in RCC tissues and paired healthy tissues by reverse transcriptionquantitative polymerase chain reaction. The results revealed that miR242 was upregulated in RCC tissues and ACHN, 786O and 769P cell lines compared with healthy tissues and HEK293T cells, respectively, whereas miR241 was almost absent in RCC and healthy kidney tissues. To investigate the role of miR242 in RCC, a synthesized miR242 mimic, negative control (NC), inhibitor or inhibitor NC was transfected into 786O and ACHN RCC cells, and cell proliferation, mobility and apoptosis assays were performed. The results of the present study revealed that miR242 was associated with cell proliferation, migration, invasion and apoptosis, thus demonstrating that miR242 may serve a role as an oncogene in RCC. Further studies are required to investigate the signaling pathways of miR242, and the potential of miR242 as a therapeutic target or biomarker for the early detection of RCC.
Full text:
1
Collection:
01-internacional
Health context:
6_ODS3_enfermedades_notrasmisibles
Database:
MEDLINE
Main subject:
Carcinoma, Renal Cell
/
Apoptosis
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MicroRNAs
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Kidney Neoplasms
Type of study:
Prognostic_studies
/
Risk_factors_studies
/
Screening_studies
Limits:
Adult
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Aged
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Female
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Humans
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Male
/
Middle aged
Language:
En
Journal:
Mol Med Rep
Year:
2017
Document type:
Article