T cell recognition of Mycobacterium tuberculosis peptides presented by HLA-E derived from infected human cells.
PLoS One
; 12(11): e0188288, 2017.
Article
in En
| MEDLINE
| ID: mdl-29176828
ABSTRACT
HLA-E is a non-conventional MHC Class I molecule that has been recently demonstrated to present pathogen-derived ligands, resulting in the TCR-dependent activation of αß CD8+ T cells. The goal of this study was to characterize the ligandome displayed by HLA-E following infection with Mycobacterium tuberculosis (Mtb) using an in-depth mass spectrometry approach. Here we identified 28 Mtb ligands derived from 13 different source proteins, including the Esx family of proteins. When tested for activity with CD8+ T cells isolated from sixteen donors, nine of the ligands elicited an IFN-γ response from at least one donor, with fourteen of 16 donors responding to the Rv0634A19-29 peptide. Further evaluation of this immunodominant peptide response confirmed HLA-E restriction and the presence of Rv0634A19-29-reactive CD8+ T cells in the peripheral blood of human donors. The identification of an Mtb HLA-E ligand that is commonly recognized may provide a target for a non-traditional vaccine strategy.
Full text:
1
Collection:
01-internacional
Health context:
3_ND
Database:
MEDLINE
Main subject:
Peptides
/
Tuberculosis
/
Histocompatibility Antigens Class I
/
Antigen Presentation
/
CD8-Positive T-Lymphocytes
/
Mycobacterium tuberculosis
Limits:
Adult
/
Humans
Language:
En
Journal:
PLoS One
Year:
2017
Document type:
Article