Investigating the structural integrity of Bovine serum albumin in presence of newly synthesized metallosurfactants.
Colloids Surf B Biointerfaces
; 164: 116-124, 2018 Apr 01.
Article
in En
| MEDLINE
| ID: mdl-29413588
ABSTRACT
Bovine Serum Albumin is major transport protein and is often used as a drug carrier in body organs. Knowledge of its binding with metallosurfactant can significantly influence the biodistribution of metallodrugs. Current work demonstrated a facile method to prepare four different double chained metallosurfactants containing Fe, Co, Ni and Cu as part of their counter ion. The as-synthesized metallosurfactants were characterized using FTIR, AAS, TGA and XRD in solid form. The aggregation of these metallosurfactants in aqueous medium was investigated through conductivity, surface tension and SAXS. Further, we have investigated their binding with BSA through different analytical methods The effect of concentration of metallosurfactants on the primary and secondary structure of BSA was further examined by SDS-PAGE and Circular dichroism, respetively. It is found that at premicellar concentration, the primary structure of BSA was not affected but the secondary structure i.e. α-helical structure of BSA was altered as shown by circular dichroism. Interestingly, post micellar concentration of metallosurfactants shows the pronounced effect on the primary and secondary structure of BSA. SAXS study also supports the fact of unfolding of protein and its wrapping around the micelles. Zeta potential describes the electrical charge and stability of the protein in the presence of different concentration of metallosurfactant. Along with, it was found that presence of protein delays the aggregation behavior of metallosurfactant, as a sign of binding of BSA with metallosurfactant.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Surface-Active Agents
/
Serum Albumin, Bovine
/
Metals
Limits:
Animals
Language:
En
Journal:
Colloids Surf B Biointerfaces
Year:
2018
Document type:
Article