Deleterious effects of calcium indicators within cells; an inconvenient truth.
Cell Calcium
; 73: 82-87, 2018 07.
Article
in En
| MEDLINE
| ID: mdl-29689523
ABSTRACT
The study of cellular Ca2+ signalling is indebted to Roger Tsien for the invention of fluorescent indicators that can be readily loaded into living cells and provide the means to measure cellular Ca2+ changes over long periods of time with sub-second resolution and microscopic precision. However, a recent study [1] reminds us that as useful as these tools are they need to be employed with caution as there can be off-target effects. This article summarises these recent findings within the wider context of confounding issues that can be encountered when using chemical and genetically-encoded Ca2+ indicators, and briefly discusses some approaches that may mitigate against misleading outcomes.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Membrane
/
Calcium
/
Calcium Signaling
/
Indicators and Reagents
Limits:
Animals
/
Humans
Language:
En
Journal:
Cell Calcium
Year:
2018
Document type:
Article