Pyroptosis induced by enterovirus A71 infection in cultured human neuroblastoma cells.
Virology
; 521: 69-76, 2018 08.
Article
in En
| MEDLINE
| ID: mdl-29886343
ABSTRACT
Enterovirus A71 (EV-A71) infection can cause hand, foot and mouth disease (HFMD), and even fatal meningoencephalitis. Unfortunately, there is currently no effective treatment for EV-A71 infection due to the lack of understanding of the mechanism of neurological diseases. In this study, we employed SH-SY5Y human neuroblastoma cells to explore the roles of caspase-1 in neuropathogenesis. The expression and activity of caspase-1 were analyzed. The potential immuneconsequences mediated by caspase-1 including cell death, lysis, DNA degradation, and secretion of pro-inflammatory were also examined. We found the gene expression levels of caspase-1, IL-1ß, IL-18 and active caspase-1 were markedly increased in the SH-SY5Y cells at 48â¯h post EV-A71 infection. The cell death, lysis, and DNA degradation were also increased during infection, which could be significantly alleviated by caspase-1 inhibition. These observations provided additional experimental evidence supporting caspase-1-mediated pyroptosis as a novel pathway of inflammatory programmed cell death.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Caspase 1
/
Enterovirus A, Human
/
Pyroptosis
/
Hand, Foot and Mouth Disease
Limits:
Humans
Language:
En
Journal:
Virology
Year:
2018
Document type:
Article