A noncanonical amino acid-based relay system for site-specific protein labeling.
Chem Commun (Camb)
; 54(52): 7187-7190, 2018 Jun 26.
Article
in En
| MEDLINE
| ID: mdl-29896591
Genetically site-specific introduction of noncanonical amino acids (ncAAs) for protein conjugation generally requires incorporation through exogenous feeding of chemically synthesized ncAAs. We developed a p-amino-phenylalanine (pAF)-based relay system that enables site-specific functionalization of proteins without chemical synthesis of the building blocks. pAF was biosynthesized under optimized conditions, followed by site-specific incorporation into a specific protein residue. The resulting protein was ready for functionalization using an oxidative conjugation reaction. We demonstrated the use of this relay system by preparing a fluorophore-labeled anti-HER2 single-chain variable fragment antibody for fluorescent imaging.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phenylalanine
/
Staining and Labeling
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Tyrosine-tRNA Ligase
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Optical Imaging
/
Fluorescent Dyes
Language:
En
Journal:
Chem Commun (Camb)
Year:
2018
Document type:
Article