Epithelial Smad4 Deletion Up-Regulates Inflammation and Promotes Inflammation-Associated Cancer.

Means, Anna L; Freeman, Tanner J; Zhu, Jing; Woodbury, Luke G; Marincola-Smith, Paula; Wu, Chao; Meyer, Anne R; Weaver, Connie J; Padmanabhan, Chandrasekhar; An, Hanbing; Zi, Jinghuan; Wessinger, Bronson C; Chaturvedi, Rupesh; Brown, Tasia D; Deane, Natasha G; Coffey, Robert J; Wilson, Keith T; Smith, J Joshua; Sawyers, Charles L; Goldenring, James R; Novitskiy, Sergey V; Washington, M Kay; Shi, Chanjuan; Beauchamp, R Daniel

Means, Anna L; Freeman, Tanner J; Zhu, Jing; Woodbury, Luke G; Marincola-Smith, Paula; Wu, Chao; Meyer, Anne R; Weaver, Connie J; Padmanabhan, Chandrasekhar; An, Hanbing; Zi, Jinghuan; Wessinger, Bronson C; Chaturvedi, Rupesh; Brown, Tasia D; Deane, Natasha G; Coffey, Robert J; Wilson, Keith T; Smith, J Joshua; Sawyers, Charles L; Goldenring, James R; Novitskiy, Sergey V; Washington, M Kay; Shi, Chanjuan; Beauchamp, R Daniel.

Affiliation

Means AL; Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee.
Freeman TJ; Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, Tennessee.
Zhu J; Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, Tennessee.
Woodbury LG; Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee.
Marincola-Smith P; Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, Tennessee.
Wu C; Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee.
Meyer AR; Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee.
Weaver CJ; Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee.
Padmanabhan C; Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, New York.
An H; Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee.
Zi J; Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, Tennessee.
Wessinger BC; Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee.
Chaturvedi R; Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee.
Brown TD; Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee.
Deane NG; Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee.
Coffey RJ; Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee.
Wilson KT; Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee.
Smith JJ; Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee.
Sawyers CL; Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee.
Goldenring JR; Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, Tennessee.
Novitskiy SV; Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee.
Washington MK; Veterans Affairs Tennessee Valley Healthcare System, Nashville, Tennessee.
Shi C; Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, Tennessee.
Beauchamp RD; Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee.

Cell Mol Gastroenterol Hepatol ; 6(3): 257-276, 2018.

Article in En | MEDLINE | ID: mdl-30109253

Subject(s)

Carcinoma/immunology; Colitis/immunology; Colorectal Neoplasms/immunology; Inflammation/immunology; Smad4 Protein/immunology; Animals; Bone Morphogenetic Protein 2/genetics; Bone Morphogenetic Protein 2/metabolism; Carcinoma/etiology; Carcinoma/pathology; Cell Line; Cell Line, Tumor; Colitis/chemically induced; Colitis/complications; Colorectal Neoplasms/etiology; Colorectal Neoplasms/pathology; Dextran Sulfate/pharmacology; Humans; Inflammation/chemically induced; Inflammation/complications; Intestinal Mucosa/drug effects; Intestinal Mucosa/immunology; Mice; Mice, Inbred C57BL; Mice, Transgenic; Smad4 Protein/genetics; Transforming Growth Factor beta1/genetics; Transforming Growth Factor beta1/metabolism

Key words

AOM, azoxymethane; APC, adenomatous polyposis coli; BMP, bone morphogenetic protein; CAC, colitis-associated carcinoma; CCL20, Chemokine (C-C motif) ligand 20; CRC, colorectal cancer; CRISPR/Cas9, Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9; Colitis-Associated Carcinoma; DMEM, Dulbecco's modified Eagle medium; DSS, dextran sodium sulfate; FBS, fetal bovine serum; FDR, false discovery rate; GFP, green fluorescent protein; HBSS, Hank's balanced salt solution; IBD, inflammatory bowel disease; IL, interleukin; IMCS4fl/fl, immortalized mouse colonoctye cell line with loxP-flanked Smad4 alleles; IMCS4null, immortalized mouse colonocyte cell line with deletion of the Smad4 alleles; LPS, lipopolysaccharide; PBS, phosphate-buffered saline; PE, phycoerythrin; R-SMAD, Receptor-SMAD; SFG, retroviral vector; STAT3, signal transducer and activator of transcription 3; TGFß; TGFß, transforming growth factor ß; TNF, tumor necrosis factor; Tumor Necrosis Factor; UC, ulcerative colitis; WNT, wingless-type mouse mammary tumor virus integration site; YAMC, young adult mouse colon epithelial cells; mRNA, messenger RNA; sgRNA, single-guide RNA

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Carcinoma / Colorectal Neoplasms / Colitis / Smad4 Protein / Inflammation Type of study: Etiology_studies / Risk_factors_studies Limits: Animals / Humans Language: En Journal: Cell Mol Gastroenterol Hepatol Year: 2018 Document type: Article

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