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Prolonged DNA hydrolysis in water: A study on DNA stability.
Fattorini, Paolo; Marrubini, Giorgio; Bonin, Serena; Bertoglio, Barbara; Grignani, Pierangela; Recchia, Elisa; Pitacco, Paola; Procopio, Francesca; Cantoni, Carolina; Pajnic, Irena Zupanic; Sorçaburu-Cigliero, Solange; Previdere, Carlo.
Affiliation
  • Fattorini P; Department of Medicine, Surgery and Health, University of Trieste, Italy.
  • Marrubini G; Department of Drug Sciences, University of Pavia, Italy.
  • Bonin S; Department of Medicine, Surgery and Health, University of Trieste, Italy.
  • Bertoglio B; Department of Public Health, Experimental and Forensic Medicine, Section of Legal Medicine and Forensic Sciences, University of Pavia, Italy.
  • Grignani P; Department of Public Health, Experimental and Forensic Medicine, Section of Legal Medicine and Forensic Sciences, University of Pavia, Italy.
  • Recchia E; Department of Medicine, Surgery and Health, University of Trieste, Italy.
  • Pitacco P; Department of Medicine, Surgery and Health, University of Trieste, Italy.
  • Procopio F; School of Biological and Chemical Sciences, Queen Mary University of London, United Kingdom.
  • Cantoni C; CNR-ISMAR, Trieste, Italy.
  • Pajnic IZ; Institute of Forensic Medicine, Faculty of Medicine, University of Ljubljana, Slovenia.
  • Sorçaburu-Cigliero S; Department of Medicine, Surgery and Health, University of Trieste, Italy.
  • Previdere C; Department of Public Health, Experimental and Forensic Medicine, Section of Legal Medicine and Forensic Sciences, University of Pavia, Italy.
Data Brief ; 20: 1237-1243, 2018 Oct.
Article in En | MEDLINE | ID: mdl-30238033
This work provides a protocol for the in vitro production of damaged DNA samples. In particular, heat-mediated hydrolysis of the samples at 70 °C in ultrapure water was performed in 1.7 mL Eppendorf tubes sealed by Parafilm for 0-36 h. The chemical/physical features of the resulting samples are described. After normalization of the qPCR data, these were compared with those obtained from samples treated for 0-10 h in a previous study.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Data Brief Year: 2018 Document type: Article
Fulltext
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PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Data Brief Year: 2018 Document type: Article