Characterisation of rotavirus isolates from sub-clinically infected calves by genome profile analysis.
Vet Microbiol
; 13(1): 27-34, 1987 Jan.
Article
in En
| MEDLINE
| ID: mdl-3027953
Rotaviruses isolated from 43 sub-clinically infected calves from a single farm were analysed by genome profile analysis. The isolates showed genomic variation and eight different profiles were observed, including one which was atypical for Group A rotaviruses. The 3' terminal labelling method for the analysis of genome profiles used in this study required only 1 ng of viral RNA, an increase of 1000-fold in sensitivity over ethidium bromide staining for detecting all rotavirus genome segments. However, dual infections involving two rotaviruses with distinct profiles could not be detected if the concentrations of the viruses differed by greater than 10-fold.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Rotavirus Infections
/
RNA, Viral
/
Cattle Diseases
/
Rotavirus
/
Genes, Viral
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Vet Microbiol
Year:
1987
Document type:
Article