Dynamic Light Scattering Analysis to Dissect Intermediates of SNARE-Mediated Membrane Fusion.
Methods Mol Biol
; 1860: 53-69, 2019.
Article
in En
| MEDLINE
| ID: mdl-30317498
ABSTRACT
Dynamic light scattering (DLS) spectroscopy provides rapid information on the size distribution of a large number of particles in a mixture. Vesicle sizes change during the merger of lipid bilayers, and DLS analysis can provide rapid, accurate, and non-perturbative quantification of the size distribution of proteoliposomes in SNARE-dependent membrane fusion. In this chapter, we describe the methodologies and reagents used for DLS spectroscopy in a biochemical and biophysical study of SNARE-mediated membrane fusion.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
SNARE Proteins
/
Dynamic Light Scattering
/
Membrane Fusion
Language:
En
Journal:
Methods Mol Biol
Year:
2019
Document type:
Article